Complete Genetic Engineering of Withania somnifera (Ashwagandha) to enhance anticancer with anolides (Theory)

In this short course, we will cover the below items:

    1. Procurement of sample

    2. Selection of molecule targeted for enhancement

    3. Surface sterilization of plant tissue

    4. Inoculation

    5. Agrobacterium infection

    6. Media preparation

    7. Media manipulations

    8. Hairy root growth and subculture

    9. Hairy roots drying

   10. Extraction of compounds from hairy roots

   11. HPLC analysis